Course 4: Next generation microbial sequence (NGS) analysis using QIIME2

Description: This course teaches students how to use the QIIME2 tool to perform Next-Generation Microbial Sequence Analysis (NGS). In this session, we will go through DNA extraction, PCR amplification, and barcoded sequencing in-depth (no hands-on). We will utilize publicly available human microbiome samples from two individuals at four body sites and five-time points for QIIME2 analysis. ESRs will learn the hands-on experience of importing data files into QIIME2 and the execution of quality filtering sequences, diversity analysis using multiple alpha and beta diversity measures, taxonomic assignments, and relative quantification of bacteria.  ESRs will be able to do microbial analysis using the QIIME2 tool as a result of this training. Microbial sequence analysis. 1 ECTS